The fruit fly serves as an excellent model for studying oogenesis, or female gamete formation. Each egg develops from an egg chamber, which progresses through the stages of oogenesis as part of a developmental array known as an ovariole. At the anterior of each ovariole lies the germarium, which contains the germline and somatic stem cells which will divide to give rise to each newly formed egg chamber. Much has already been learned about oogenesis using a combination of fluorescence and electron microscopy to study specific structures and proteins; however, a complete picture of the organization of germline and somatic cells and their intracellular structures and organelles is lacking. To learn more about the early stages of oogenesis, we are rendering a large data set collected using Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) to generate a complete 3D model of the germarium and an early-stage egg chamber. Our lab is primarily interested in the structure of the germline ring canals, or intercellular bridges, which allow nurse cells to transfer their cytoplasmic contents into the growing oocyte. Electron microscopy has previously revealed the presence of extensive membrane protrusions, or microvilli, surrounding the germline ring canals.This microvilli meshwork is thought to maintain the anchoring and stability of the ring canals during egg chamber growth, yet the stage of oogenesis when these protrusions first appear and their spatial distribution within the germline is not known. Therefore, we hope to characterize changes in the size and structure of the germline ring canals and the organization of germ cell membranes during early oogenesis. Once completed, we envision that this dataset can become a valuable resource that can be further mined by other researchers in the field.